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Both tissue and cellular RNA were extracted using the TRIzol reagent (15596026CN, Invitrogen) following the manufacturers protocol

Laboratory Safety: Use standard laboratory safety practices, including sterile technique and appropriate personal protective equipment (PPE) during handling and reconstitution

In vitro assays revealed the interaction of TNBS with GSH leading to the almost instantaneous disappearance of GSH, while the reductive metabolism of TNBS by GSSG reductase generated ROS

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Thus, interference with GSH synthesis in FoB induced an increase in mitochondrial mass without concomitant mtDNA replication

This reaction can occur both non-enzymatically and through catalysis by glutathione peroxidase [14, 15]