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Q-PCR reaction mixtures contained 1 l cDNA, 1x SYBR Green Supermix (BioRad, Hercules, CA), and 0.15 M gene-specific primer sets in a total volume of 25 l

Another exploration in this field showed advanced glycation end products (AGEs) to be potential biomarkers of OS in individuals with HT

Both groups then received an injection of fentanyl (75 g/kg, IV) and BT was recorded 5, 10, 15, 20, 25 and 30 min later

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