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Quantitative Real-time-PCR (qRT-PCR) analysis The relative ovarian SIRT1, steroidogenic acute regulatory protein (STAR), cytochrome P450 17A1 (CYP17A1), hydroxysteroid (17) dehydrogenase type 3 (HSD17B3), nuclear factor erythroid 2 related factor 2 (Nrf2), mitochondrial DNA (mtDNA), forkhead box protein O1 (FOXO1), nuclear factor kappa B (NF-B), and inducible nitric oxide synthase (iNOS) mRNA abundance was determined by qRT-PCR using SYBR Green PCR Master Mix (2x SensiFast SYBR, Bioline, catalog No

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This biochemical tweak allows it to cross the blood-brain barrier , making it not only useful for energy but also for brain health

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These individuals cannot efficiently convert cyanocobalamin to the active methylcobalamin form, which is required for methionine synthase activity in the methyl donor cycle