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Blastocyst formation rate: Defined as the proportion of 2PN zygotes (not just of cleaved zygotes) which are at the blastocyst stage at Day 5 (116 2 h post-insemination) (18), We employed the Gardner system to evaluate the quality of blastocysts in terms of their expansion, inner cell mass, and trophoblast development (20)

Plank LD, Mathur S, Gane EJ, et al

Ferroptosis, a new form of cell death: Opportunities and challenges in cancer

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Briefly, Equal number of cultured cells was collected and the cell pellet was immediately frozen and stored in liquid nitrogen for subsequent ATP analyses