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Lysates were loaded onto NuPAGE 412% Bis-Tris acrylamide gels (1.0 mm, 10-well, Invitrogen), electrophoresed with MES running buffer and then transferred onto 0.45 m PVDF membrane by electroblot using a Mini Blot module (Invitrogen)

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AC: Conceptualization, Supervision, Writing original draft, Writing review & editing

Standard Dose The most common beginner dose is 1 mg per day, injected subcutaneously in the abdominal area

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Defects in the mechanisms that ensure mitochondrial viability, such as mitophagy, are associated with numerous disease states, in particular neurodegenerative diseases such as Parkinson disease (PD)