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Description
As previously mentioned, inhibitors targeting NNMT often bear structural resemblance to one or both of its substrates
Equal amounts of cell lysates were prepared using a protein loading buffer, and the samples were homogenized to a final concentration of 2 g/L
Recombinant proteins were then purified by affinity chromatography using glutathione Sepharose 4B (GE Healthcare)
10.1136/openhrt-2015-000300 182
males) were distributed across ten 700-l tanks supplied with SW for the FW transfer experiment
Wound care to prevent infection
