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We next investigated whether the glucagon protein level and glucagon granule number were altered in GCGR-KO mice

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Whether chronic morphine exposure induces constitutive activity of MOR, or whether this is widespread in the nervous system is therefore still uncertain, but if correct, the observation that constitutive MOR activity increases after chronic opioid exposure suggests an active confomational state of MORs that is evident even in the absence of bound ligand