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An additional 700 l of 1X Lysis/Binding/Wash Buffer with a total of 1.5 mg protein was added to the beads in each tube and incubated overnight at 4 C with gentle rotation
Briefly, HT-1080 cells were seeded into 15-cm dishes at a density of 1 10 6 cells/dish, then the next day treated nutlin-3 (10 M)
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10.1152/physrev.00026.2013 203 ZouM
Detoxification Support: Provides the liver with extra tools during its most active hours
[DOI] [PMC free article] [PubMed] [Google Scholar] 107.Ballatori N., Rebbeor J
