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During escalation around weeks 16 to 20, the peak ran two to three times higher

ROS damages oocyte spindle microtubules and also blocks formation of new spindle microtubules ( 65 )

After loading 3 l of sample onto a C8 column at 40 C, elution was performed by the following gradient: 20% solvent B for 2 min, followed by a linear increase to 100% solvent B within 5 min, then maintaining 100% B for 33 min, then returning to 20% B in 1 min

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