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Briefly, 8000 cells per well were plated into 96-well plates (Corning Falcon) and allowed to attach to the plate overnight

Scars close wounds effectively but differ from surrounding tissue in collagen organization, cellularity, vascularity, and function

34 Isoform-level TPM values were used as input to compute percent spliced-in (PSI) values for each AS event, including exon skipping (SE), alternative 5/3 splice sites (A5/A3), mutually exclusive exons (MX), retained introns (RI), and alternative first/last exons (AF/AL)

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Understanding these developmental influences can help identify individuals who may be at a higher risk for ACL injuries based on their anatomical characteristics

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