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Cells were manually scraped, spun at a 100 g for 5 min, the pellets washed with PBS and then homogenized in a Tris salt buffer, transferred to ultracentrifuge tubes and spun at 163, 200 g and 4 C for 30 min to extract the soluble fraction

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They may also improve insulin function and slightly lower blood pressure in those with PCOS ( Whats more, preliminary research found that the combination of inositol and folic acid may promote ovulation in women with fertility issues from PCOS ( In one study, 4 grams of inositol and 400 mcg of folic acid taken daily for 3 months induced ovulation in 62% of treated women ( Summary Inositol may help reduce blood triglyceride levels, improve insulin function, lower blood pressure and promote ovulation in women with polycystic ovary syndrome (PCOS)

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