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This kind of precipitation allows resuspension of the protein pellet in isoelectric focusing buffer contains 8 M urea, 2 M thiourea, 10 mM 1,4-dithiothreitol (DTT), 2 mM TCEP (Tris(2-carboxyethyl)-phosphine-hydrochloride), 1% (w/v) CHAPS, 1% (w/v) Triton X-100, 1% (w/v) amidosulfobetaine-14 (ASB), and 0.4% (v/v) ampholytes (pH 310)