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Water content was calculated according to the following formula: [(wet weight dry weight)/(wet weight) 100 (%)] ( Evaluation of Neurological Deficits Neurological deficits were evaluated using a five-point scale established by Immunohistochemistry and Nissl Staining Immunohistochemistry experiments were performed using the UltraSensitiveTM SP (Mouse/Rabbit) immunohistochemistry Kit (Mixim, Fu Zhou, China), following the manufacturers instructions