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The GST cDNA sequence from pGEX-6P-2 (GE Healthcare) was PCR amplified with primers containing NcoI and KpnI restriction sites at the 5' and 3' extremities, respectively

GSH is the most abundant non-protein thiol that functions as an antioxidant and redox regulator in cells

High levels of oxidative stress surpass the defence capability of the antioxidant response, and induce mitochondrial permeability transition pores and disruption of the electron transfer, thereby resulting in apoptosis or necrosis.11 Nrf2 is a key transcription factor of the hepatic adaptation to sustained oxidative stress

[DOI] [PMC free article] [PubMed] 70.Koleini N, Shapiro JS, Geier J, Ardehali H

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doi: 10.1186/s12916020-01587-w 202 TraberMGStevensJF