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Method Development of an In Vitro Vertical Franz Diffusion Cell System to Assess Permeation of Cosmetic Active Ingredients

Hippocampal cell culture preparation Hippocampal neurons (2xl0 5 cells per square cm) were cultured from PI Sprague Dawley rats on plates coated with poly-L-lysine from Sigma (St

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61 , e202112236 (2022)

Add directly to culture medium at final concentrations of 0.110 mM, depending on assay sensitivity

S.MilesL.ReaumeJ.et al (2011)
