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In human OA cartilage, ACLT-induced models and IL-1-stimulated chondrocytes, both ACSL4 and lipid peroxidation marker 4-HNE are elevated

Autophagy and mitophagy have also been implicated as potential contributors to therapeutic resistance, with increased expression of mitophagy-related proteins such as PINK1 reported to be associated with poor response to NAC [8,9,10]

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Afterward, the slides were washed with PBS three times and then incubated with secondary antibodies for 30 min

This ensures that your body receives more of the active compound where it is needed most