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After incubation with the primary antibodies, the membranes were probed with the corresponding horseradish peroxidase-conjugated secondary antibodies (1:10,000 dilution

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They are N -trimethyllysine hydroxylase, 3-hydroxy- N -trimethyllysine aldolase, 4- N -trimethylaminobutyraldehyde dehydrogenase and -butyrobetaine hydroxylase

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L-aspartic acid (L-Aspartate), the L isomer of the aspartic acid, is one of twenty three proteinogenic amino acids