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These metabolites include blood levels of lactate and pyruvate.[38] Blood levels of magnesium should also be checked because magnesium is required for the conversion of thiamine into its active form, thiamine pyrophosphate.[39] A presumptive diagnosis of WE can be confirmed by determining blood thiamine concentrations or by measuring the erythrocyte transketolase activity.[40,41] This test is limited by its lack of specificity.[42] Recently, an isocratic high performance liquid chromatography method for the assessment of thiamine, thiamine monophosphate, and thiamine diphosphate in human erythrocytes has been described.[43] This procedure has improved reproducibility, practicability, and performance compared with previous methods, and is suitable for both clinical and research purposes

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Elashiry M, Morandini AC, Cornelius Timothius CJ, Ghaly M, Cutler CW

Thiamine deficiency induces oxidative stress and exacerbates the plaque pathology in Alzheimer's mouse model

Oleuropein improves mitochondrial function to attenuate oxidative stress by activating the Nrf2 pathway in the hypothalamic paraventricular nucleus of spontaneously hypertensive rats