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This was related to insulins ability to induce GCLC expression (GCLM was unchanged as in hepatocytes)

Apoptosis assay Apoptosis was detected using the Annexin V-FITC Apoptosis Detection Kit (Beyotime) according to the manufacturers instructions

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Enfin, la synthse de glutathion est troitement lie la quantit de cystine disponible au sein des cellules