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During the stress response, GAPDH is translocated to the mitochondria, where it transnitrosylates mitochondrial proteins such as Hsp60, acetyl-CoA acetyltransferase (ACAT1), and voltage-dependent anion channel 1 (VDAC1) [119]

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This method involved blocking free thiol bonds, enriching SNO sites with solid-phase resin, and purifying peptide segments with isotope or isobaric labeling [93]

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