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Peak alignment, band-broadening correction and normalisation procedures were performed by selecting the central 50% region of the peaks

10.1016/j.semcdb.2012.03.014 49

The Boc-protected cystine-1,1- 13 C was reacted with two equivalents of glycine tert -butyl ester (OtBu) hydrochloride using 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide monohydrochloride and 1-hydroxybenzotriazole to produce the N and C-protected dipeptidyl (Boc-Cys-Gly-OtBu) disulfide derivative

Store at room temperature out of direct sunlight
The GluCEST approach has promising potential as it offers good spatial and temporal resolutions and covers a full brain slice to yield a color map reflective of Glu concentration

Sloan et al., 2018
