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The ethanol extract (0.25 mL) was mixed with 3 mL of anthrone reagent, incubated at 100 C for 10 min, cooled to room temperature, and absorbance at 630 nm was recorded using a spectrophotometer (SpectramaxPlus 384, Molecular Devices, USA) to estimate the sugar content

Similar results were observed in H358 cells ( Figure 1e, g

Both losses of normal cellular function and gain of aberrant toxic functions (or both together) are implicated as pathogenic mechanisms in ALS, depending on the protein involved

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