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Histopathological examination For light microscopic investigations, gastric samples were fixed in a 10% formaldehyde solution, processed by routine paraffin embedding technique

Following a washing step, Alexa Fluor 594-conjugated goat anti-rabbit IgG (1:400, Molecular Probes, Waltham, MA, USA) and/or Alexa Fluor 488-conjugated goat anti-mouse IgG (1:400, Molecular Probes) were/was added as the secondary antibody and DAPI was used for nuclear staining

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The ability to integrate 2D thermal proteome profiling will merge temperature dependent and isothermal ligand concentration-dependent studies to address prevailing false negatives associated with CETSA-MS

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