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Tagged proteins were competitively eluted with Pierce 3x DYKDDDDK peptide and analyzed by western blot ( A ), Pierce Renilla Luciferase Glow Assay ( B ), and silver staining ( C )
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Intracellular GPX4 activity detection Initially, EJ cells (5 10 5 ) were cultured in 6-well plate with a CO 2 incubator overnight at 37 C
