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Mitochondrial ROS were stained with MitoTracker Red CM-H 2 X (Invitrogen) as described in 71

8 weeks (1 vial): 3 mL 1 10 mL bottle 12 weeks (2 vials): 6 mL 1 10 mL bottle 16 weeks (4 vials): 12 mL 2 10 mL bottles Alcohol Swabs One for the vial stopper + one for the injection site each week

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