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Testis protein extraction was performed using T-PER Tissue protein extraction buffer (Thermo Scientific, Waltham, MA, United States) and Halt Protease and Phosphate inhibitor cocktail (Thermo Scientific) following the manufacturers recommendation
Furthermore, using both inhibitors and RNA silencing we have shown that TGR is an essential schistosome protein, and we believe that our results have validated it as a key drug target for treatment of schistosomiasis

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10.1016/j.arr.2023.101936 [DOI] [PubMed] [Google Scholar] Wan X., Garg N

Macrophage Recruitment The peptide also acts as a chemoattractant for macrophages, particularly the M2 polarization associated with the proliferative phase of repair