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Fluorescence polarization anisotropy assay Fluorescence polarization assays were conducted using a Cytation 5 cell imaging multi-mode reader (BioTek) equipped with a green FP filter at 25 C, utilizing 96-well microplates (SPL 30496)

Stability evaluation Evaluate lyophilizate stability under controlled storage and reconstitution conditions

Magnesium and potassium aspartate further support muscle activity and metabolic balance

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