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To inhibit autophagy, vitiligo melanocytes were grown in M254 containing HMGS and treated with the autophagy inhibitor 3-methyl adenine (5 mM) (Sigma Aldrich) for 24-48-72-96 h whereas vitiligo fibroblasts were cultured in DMEM with 10% FBS and incubated with the inhibitor 3-methyl adenine (5 mM) (Sigma Aldrich) for 24 and 48 h
Induction of apoptosis in human breast cancer by adenoviral mediated overexpression of the proapoptotic gene bax

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