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The principle is straightforward: a purified bait protein is immobilized on an affinity matrixtypically through a fusion tag or high-specificity ligandand then incubated with a protein mixture containing potential binding partners (prey)

The appropriate complex consists of the name-giving Nox subunit and the smaller p22phox, which stabilizes the Nox protein

doi: 10.1016/S0168-6445(03)00046-9

Results Enhanced NRF2 signaling correlates with poor survival rates in patients with HNSCC The NRF2 degradation complex is crucial in the regulation of NRF2 signaling and comprises KEAP1 and CUL3 components

[PUBMED Abstract] Aric M, Valsecchi MG, Camitta B, et al.: Outcome of treatment in children with Philadelphia chromosome-positive acute lymphoblastic leukemia

Ascorbyl radicals are relatively stable and have very limited reactivity compared to the primarily formed radicals in the cascade