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The Glass labs results in aged CTX-injured animals may reflect the severe depletion of regenerative stem cells in this model

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For proliferation analysis, expanded HBs were fixed with 4% PFA, incubated in blocking and permeabilizing buffers (0.1% Triton X-100, 5% normal donkey serum), and then incubated with APC-conjugated Ki67 and other indicated antibodies [1]

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