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Suppression of high-fat-diet-induced obesity in mice by dietary folic acid supplementation is linked to changes in gut microbiota

7 Annual Report on the Aging Society, 2020 8 Grants-in-Aid for Scientific Research, MHLW Special Research Project

(2011) proposed a sandwich layer method in which colonies from soil samples are first cultured on medium A, then the whole agar of medium A is lifted from the plate and transferred to the surface of medium B (containing the methylene blue indicator), thus forming a medium A-colonies- medium B sandwich. After incubation for 2 h at 40C, strains forming haloes are then picked and purified

Additional metabolic panels may be ordered periodically to monitor for any effects on glucose metabolism, liver function, or other parameters

There is no clear relationship between the oral bacterial community and lung function in healthy organisms, but changes in the oral microbiota may affect lung disease because the lungs are exposed to the oral microbial community through respiratory movements such as breathing and coughing

F.KruegerN.et al (2018)