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Next, the membrane was incubated with electrochemiluminescence substrate (#1705060, Bio-Rad, Hercules, CA, USA) for 5 min and analyzed using FluorChem M (ProteinSimple, Santa Clara, CA, USA)

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As PD is a multisystem disorder with a long prodromal phase affecting autonomic, bowel, bladder and other systems, incorporating multimodal information from the wider body could be particularly informative for earlier diagnosis, and as a measure of progression

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