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10.1113/JP271957 J

doi: 10.1084/jem.20040624 117 WalmsleySRPrintCFarahiNPeyssonnauxCJohnsonRSCramerTet al

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In conditions of oxidative stress, melatonin reduces the expression of activated caspase-3 and the Bax/Bcl-2 ratio, preventing neuronal apoptosis [50,99,100,101]

After 180 min of anaerobic incubation at 37 C, the entire culture volume was collected into 50 mL conical tubes with pipetting and scraping to capture adherent cells, and the cells were centrifuged at 4000 g for 10 min, yielding pellets with a wet weight of ~100 mg