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And the 96-well plates were incubated for another 3 h at 37 C and followed by determining the absorbance at 450 nm utilizing a microplate reader (Thermo, USA)

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Both GSH and SB431542 significantly down-regulated the TGF1/SMAD pathway and the expression of -SMA under high glucose conditions (Fig

3A and B), and chondrogenic marker expression was significantly increased in the three MSC types with high GSH levels, along with a significant decrease in COLXA1 expression (Fig

In these individuals, decline in functional independence from baseline may be a more appropriate indicator of stage

Diaz-Gil, D