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Cells were permeabilized using digitonin (1 g per 10 6 platelets or 6 g per 10 6 PBMCs), and simultaneously, the N-pathway substrates (or CI substrates) malate and pyruvate were added at saturating concentrations (5 mM each)

Authors performed a simultaneous treatment with an autophagic inhibitor (bafilomycin) and activator (rapamycin), which unexpectedly led to decreased lipid accumulation, suggesting a novel pathway involving p62-mediated turnover of lipid droplets and the potential export of triglycerides into the extracellular space [126]

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Given the upregulation of compensatory metabolic pathways, including FSP1 and DHODH, in resistant glioblastoma cells, combinatorial inhibition of GPX4 and FSP1 represents a promising therapeutic avenue that warrants further investigation

Glucocorticoids Glucocorticoids (GCs) are steroid hormones produced by the zona fasciculata of the adrenal cortex