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Direct nicotine binding to nicotinic acetylcholine receptors on BECs induces acetylcholine-dependent nitric oxide (NO) release via activation of neurovascular endothelial NO synthase (Mazzone et al., 2010)

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Ice or over-the-counter anti-inflammatory medications, such as ibuprofen, can resolve any residual pain

For those learning the technique, training with a healthcare provider or using reliable equipment with built-in safety measures can reduce errors, prevent contamination, and improve overall confidence in the injection process

All compounds are strictly for in-vitro research applications

Research remains in early stages with no human clinical trials currently approved