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In brief, cells from nearly confluent 15-cm plates treated with 200 M H 2 O 2 for 30 min were harvested and lysed in IP buffer (20 mM HEPES, 150 mM NaCl, 2 mM MgCl 2 , 0.5 mM CaCl 2 , 1% (v/v) Brij-35, and protease inhibitor cocktail)

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55 This is how severe oxidative stress can deplete the cellular reserve of GSH, which would facilitate the progression of certain disorders associated with aging

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Shomali et al., 2022)

In line with this, our P301L-tau overexpressing cells exhibit shorter neurites but more neurites per cell and a greater number of neurite branches than the Vector cells