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DNAs were ligated into pCS2 vectors for transient expression in mammalian cells
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Primarily, TQ molecule suppresses cell proliferation through cell cycle arrest, microtubule organization disruption, and downregulation of pro-survival proteins [48]
HS024 (MC4R selective antagonist) abolishes 7278% of BDNF upregulation, confirming MC4R-CREB-BDNF cascade dependence
