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For instance, glutamine dose-dependently enhanced cell proliferation and differentiation of neonatal porcine and rat IECs, through activating multiple mitogen-activated protein kinases (MAPKs) (108110)
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The Pen a 1 mutant VR9-1, carrying 12 amino acid substitutions across major IgE-binding epitopes, demonstrated a remarkable 90%98% reduction in allergenic potency, suggesting its potential as a therapeutic agent in shellfish immunotherapy [149]

Design issues include study objective, availability of data, sampling methods, data collection, cost of the design, time implications, controls, and ethical considerations (Wang and Kattan 2020)

Lu et al., 2025)
