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Western analysis Whole cell extracts from primary myoblasts or C2C12 cells were resolved on a 15% SDS-PAGE gel, transferred to Polyvinylidene difluoride (PVDF) membrane (Bio-Rad), and probed with the following primary antibodies: C/EBP (E299, ab32358, Abcam), MYL (F5, sc-365243, Santa Cruz), MYL12A/B (A-10, sc-376606, Santa Cruz), MYL4 (ab231800, Abcam) and Cyclophilin B (ab16045, Abcam)
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