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After centrifugation, the supernatant was further cleaned by liquid-liquid extraction in 0.73% NaCl and methanol

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The patient continued treatment with pemigatinib for 8 months before imaging showed progression of disease

This energy easily suffices for arbitrarily large pores, even for vesicles that do not experience shear stress with membrane tension \(\sigma =0.\) This estimation shows that all vesicles in our experiments could fission, if the pore formation energy would be the single decisive parameter

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2020 Dec;13(6):65
