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Blots were blocked with phosphate buffered saline with 0.1% Tween 20 and then binding with antibodies for eNOS (1: 1000) or caveolin-1 (1: 1000), followed by horseradish peroxidase-conjugated goat anti-rabbit (diluted 1: 5000) and enhanced chemiluminescence reaction was performed for blots development

DTP cells utilize diverse adaptive strategies to optimize the ecological niche, undergo metabolic reprogramming, and interact with microenvironment

Grosjean E, Simonneaux V, Challet E

Consistent with these findings, our data shows that A 40 oligomers can increase MMP-2 activity in stimulated astrocytes and correlated with increased oxidative stress, inflammation and reduced cell viability