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Mutated GST sequences were subcloned into pET-15b, pET-24d, pGEX 4T-1, and pCMV-2B expression vectors, and the mutated constructs were confirmed by DNA sequencing

While future studies can use either GCLC knockdown or BSO pretreatment to further investigate the relationship between BDG response and the glutathione pathway, we have chosen chemical inhibition given efficient transient gene knockdown is technically challenging human primary monocytes

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Cytokine responses in severe acute respiratory syndrome coronavirus-infected macrophages in vitro : possible relevance to pathogenesis