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Cells were lysed using a cell disruptor (Constant Systems Ltd.) at 30 Kpsi in lysis buffer (20 mM HEPES pH 8.0, 500 mM NaCl, 0.1 mM PMSF, 20 g/ml DNaseI and 1x cComplete EDTA-free protease inhibitor cocktail (Roche))

301,305 Moreover, different oxidants, particularly the less reactive ones, exhibit target selectivity, differing, e.g., in the amino acid side chains, they typically react with

Cur is a very hydrophobic drug, and its exertion is highly dependent on the local microenvironment (Lbtow et al., 2019)

[DOI] [PubMed] [Google Scholar] 36.Lencastre A, Lamas A, Sa D, Tosti A

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