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If the redox potential of a thiol/disulfide couple is to be determined, problems already show up with low molecular mass compounds such as GSH or cysteine, since they inactivate all metal electrodes (Jocelyn, E 0 or midpoint potentials at defined conditions (e.g., E m7 at pH 7) of such compounds are usually not determined directly, but estimated by means of the Nernst equation from concentration changes after equilibration with other redox couples of seemingly known standard potential (Rall and Lehninger, E m7 values ranged from 350 to +40 mV depending on the methodology applied (Rost and Rapoport, E m7 which is at present dogmatically accepted, although method sensitivity remains a problem (Van Laer et al., The experimental difficulties to obtain reliable potentials of thiol/disulfide systems prompt further concern to accept these parameters or changes thereof as critical determinants of biological events

PMC 3547917

Anti-Aging: Combat the signs of aging by reducing oxidative stress and enhancing cellular health

Gustafsson, F

CIHR (Canada)

These findings suggest that DSIP may play a significant role in developing novel therapies for neurodegenerative diseases such as Alzheimers and Parkinsons