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The cells were washed with PBS and the fluorescence intensity of intracellular dichlorofluorescein (DCF) was measured using the FLUOstar Omega microplate reader

PMID 12185074

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They include nutritional sciences majors Theodora Afantchao , Precious Ahabue , Janyia Hightower , Mackenna Jenkins , Sydney Munnagle , and Molly Piller , dietetics major Gabriela Contreras , and dietetics & culinary science and nutrition double major Tara Goff

One innovative strategy employed the layer-by-layer assembly technique to coat liposomes loaded with IL-12 ( Figure 3B ) ( In vitro fluorescence microscopy revealed distinct behaviors: liposomes with HA as the terminal layer were effectively internalized by two cancer cell lines, whereas PLE-coated liposomes bound to the cell surface membrane without significant uptake across multiple cell types