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FDA 2006 49.Dsa E, Helta K, Radovits T, et al

The reactions were performed in a 20 L volume containing SYBR Green I Master Mix, 0.5 M forward and reverse primers, and 2 L of cDNA template

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doi: 10.1503/cmaj.060328

This differentiated status of DMSO-treated HepaRG cell cultures was routinely checked by their phase-contrast microscopy analysis, demonstrating the presence of hepatocyte-like islands and the formation of bile canaliculi, as previously reported ( 39 )