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After 5-FU treatment, PBS washing was performed once, and 100 l of fresh RPMI 1640 medium was added

This measure quantifies the difference in variability after scaling to the mean of each group

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These biomarkers allow early diagnosis, stratification, and targeted interventions in ASD [8,9,10]

However, hormones such as testosterone, estrogen, and cortisol significantly impact brain health, mood regulation, and cognitive function

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