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Fractions containing CBS protein were pooled and treated with His-tagged TEV protease overnight at 4 C and then passed over Ni-NTA agarose resin to remove the TEV protease and uncleaved protein

Ying Z, Kherada N, Farrar B, Kampfrath T, Chung Y, Simonetti O, Deiuliis J, Desikan R, Khan B, Villamena F, Sun Q, Parthasarathy S, Rajagopalan S

Brief temperature excursions during shipping (2448 hours at ambient) are tolerable, but long-term room temperature storage eliminates biological activity

Discussion In this study, we demonstrate that the M105I (hyh) mutation induces intrinsic dysfunction of -SNAP by impairing its membrane lipid binding capacity

[DOI] [PubMed] [Google Scholar] 26.Takagishi T., Hara T., Fukada T

Recent work demonstrated that S-protected thiolated -cyclodextrin (CD) derivatives increased mucoadhesion several-fold relative to native CD by enhancing both the viscosity and the interpenetration with the intestinal mucosal surface [103]