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Morphologically, nuclear alterations in ferroptosis primarily manifest as significant mitochondrial shrinkage, increased membrane density, and reduced or lost mitochondrial cristae, marking ferroptosis as a distinct form of cell death (Yan et al., 2021)

The coverslips were washed three times in PBS and once in dH 2 O, and adhered to glass slides with 23 L ProLong Gold Antifade Mountant and stored horizontally at room temperature overnight in the dark

This environment allows protonation of the binding glycoprotein and the action of enzymes to activate the binding protein to fuse the virus membrane to the surrounding membrane and release virus RNA into the cytoplasm [86]

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